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♺ Biohacking!

Logo of telegram channel biohacks — ♺ Biohacking! B
Logo of telegram channel biohacks — ♺ Biohacking!
Channel address: @biohacks
Categories: Uncategorized
Language: English
Subscribers: 286
Description from channel

Biological methods, tips and tricks and other useful stuff for the interested biohacker.
Feel free to give a little review: https://telegram.me/tchannelsbot?start=Biohacks
Contact: @Blury

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The latest Messages

2017-05-21 18:06:09 Hello biohacking-interested people!
Long time no see but never the less - here we go again!

Have you ever heard about a thing called "Paperfuge"?
Hopefully you did! If not - please continue and watch the following YouTube Video:
Paperfuge Video created by Stanford bioengineers
4.0K views15:06
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2017-01-10 02:22:05 General growth media
3.3K views23:22
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2017-01-01 03:55:51 Happy new year by the way.
2.7K views00:55
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2017-01-01 03:54:19 Bacterial transformation and GFP
2.6K views00:54
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2016-12-25 21:24:58 Merry Christmas everyone!
2.0K views18:24
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2016-12-15 00:12:00 Hydra - anti ageing
2.0K views21:12
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2016-11-25 07:59:40 DIY Centrifuge
Building your very own Centrifuge

Get a rotor
You will definitely need a rotor. Either use a Dremel or just a drill.

3D print
If you have a 3D printer yourself you can print the parts on your own. If not... Try to get someone to print the parts for you/Buy 3D prints from the web!

Ideas and Links:
• Dremelfuge
• Culinary Centrifuge
• F.Lab Centrifuge

And if you want to go really cheap...
Under $5 Centrifuge (excluding 3D pen)
1.8K views04:59
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2016-11-23 23:52:59 Cancer Gene Therapy
CRISPR/Cas9 is able to cure diseases!

AMP UP T CELLS
As simple as that the treatment can be. The T-Cells get edited to detect and survive the cancer cells which gained the ability to switch off any T cell that gets in their way.

Plan
The researchers will remove T cells from the patients and then using harmless virus to deliver the CRISPR machinery into the cells, perform three gene edits on them.

Which abilities will be granted?
• Let T cells produce a protein that sticks to cancer cells
• Remove inhibitors that interfere with this process
• Gives power to stay (removes some genetic code that allows cancer cells to detect the T cell)

Nature.com article
1.5K views20:52
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2016-11-20 01:40:45 A very nice video about CRISPR
Genetic engineering will change everything forever

Video Link
1.2K views22:40
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2016-11-19 04:06:53 Industrial production of monoclonal/polyclonal antibodies
A way to produce monoclonal/polyclonal antibodies in vitro.

Antibodies are used by your immunesystem to respond to antigenes.
To produce these we need cells that are producing antibodies for that specific antigene.

First step:
Immunization via primary injection and burst injection.
(For example you could use a mouse)

Second step:
Harvest the antibody secreting cells and fusion them using polyethyleneglycole (PEG) with a hybridoma cells.
(Polyethyleneglycole destroys the cell membrane causing the cells to fusion. The cells are now immortalized.)

Removing the PEG via dilution and centifugation
Centrifuge at 5000 G for 5 minutes.
Remove the supernatant and replenish with cell culture media with a lower concentration of PEG.
Repeat until there is close to no PEG left.

Hybridoma seperation:
Try to cultivate the fusioned cells in HAT-Media that only allows successfully fusioned cells to grow.

Screening:
Pick just one cell or many for production.
If you are using just one cell - monoclonal (Only one epitope binding place)
More than one cell - polycolnal (More than just one epitope binding places)

Choose best cells
Choose the cells that produce the most antibodies. Grow the cells in T75 flasks in HAT media to let them produce more antibodies.

Harvest antibodies:
Harvest the antibodies through cell culture media.
The cells can be threated like any other cell line.

Image Link
1.1K views01:06
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