!Investigation of pancytopenia Blood film – detailed morphol | Oxygen-Pediatry

!Investigation of pancytopenia

Blood film – detailed morphology of all cell lines
Red cell indices – the anaemia in AA is normocytic or mildly macrocytic
Reticulocytes –<20 10^9/L suggests severe aplastic anaemia
Viral titres – hepatitis, Epstein–Barr, parvovirus (usually causes red cell aplasia)
Chromosomes for breakage analysis
BM aspirate and trephine – to assess morphology and cellularity of the cells and to exclude infiltration. In AA it is hypocellular but the remaining cells are normal