Industrial production of monoclonal/polyclonal antibodies A wa | ♺ Biohacking!

Industrial production of monoclonal/polyclonal antibodies
A way to produce monoclonal/polyclonal antibodies in vitro.

Antibodies are used by your immunesystem to respond to antigenes.
To produce these we need cells that are producing antibodies for that specific antigene.

First step:
Immunization via primary injection and burst injection.
(For example you could use a mouse)

Second step:
Harvest the antibody secreting cells and fusion them using polyethyleneglycole (PEG) with a hybridoma cells.
(Polyethyleneglycole destroys the cell membrane causing the cells to fusion. The cells are now immortalized.)

Removing the PEG via dilution and centifugation
Centrifuge at 5000 G for 5 minutes.
Remove the supernatant and replenish with cell culture media with a lower concentration of PEG.
Repeat until there is close to no PEG left.

Hybridoma seperation:
Try to cultivate the fusioned cells in HAT-Media that only allows successfully fusioned cells to grow.

Screening:
Pick just one cell or many for production.
If you are using just one cell - monoclonal (Only one epitope binding place)
More than one cell - polycolnal (More than just one epitope binding places)

Choose best cells
Choose the cells that produce the most antibodies. Grow the cells in T75 flasks in HAT media to let them produce more antibodies.

Harvest antibodies:
Harvest the antibodies through cell culture media.
The cells can be threated like any other cell line.

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