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Unfortunately it's just a copy pasted protocol from a 2006 pat | ⚡ UnprecedentedNewsNet ⚡

Unfortunately it's just a copy pasted protocol from a 2006 patent on SARS and Ebola inhibitors, you would need mutagenesis primers for the gain-of-function PCR mutagenesis, the plasmid backbone and the expression vector being used, etc. and there is no reference to any of these things

https://patents.google.com/patent/WO2008045017A2/en